The perfect pipetting technique requires a combination of skill and practice. Every laboratory technician must become familiar with their instruments, know the protocols for proper sample handling, and develop an understanding of how each step influences accuracy.
If you’re looking to become a pro at accurate pipetting, here are some key tips and tricks to help you out!
Read the manual.
Before attempting to use any instrument, it is important to read and understand the instruction manual. This will give you an idea of what features your pipettor offers, as well as how to use them properly and safely.
Additionally, it is a good idea to become familiar with the standard operating procedures for pipetting in your lab.
Familiarize yourself with the pipette.
Make sure you understand the components of the pipette and how to operate it correctly, such as being familiar with the buttons or switches that control different functions like volume setting and aspiration/dispense controls.
Practice good technique.
When using a pipettor, it is important to maintain a good posture and grip on the instrument. This will help you get more precise results and reduce fatigue. Additionally, you should always aspirate and dispense slowly to avoid bubble formation or splashing of fluids.
Set the correct volume.
Before starting your pipetting procedure, be sure to set the correct volume, especially if you are using multiple samples. Setting the incorrect volume can lead to inaccurate results and wasted time.
Pay attention to accuracy.
If you are pipetting an unknown sample, it is important to pay close attention to your results. Be sure to record your results accurately and accurately track any adjustments you may have made to the pipette.
Clean your pipettes regularly.
Regular cleaning of your pipettes is important for maintaining accuracy and avoiding contamination or cross-contamination between samples. Make sure to follow the manufacturer’s instructions for proper cleaning of the instrument.
By following these steps, you can ensure that you are using the perfect pipetting technique for any sample. With practice and patience, you will soon be able to master the perfect pipetting technique.
